Deoxyribonuclease I
RRPFR0204
Deoxyribonuclease I is a nucleic acid endonuclease that digests single- or double-stranded DNA to produce monodeoxyribonucleotides or single- or double-stranded oligodeoxyribonucleotides.The product of hydrolysis of single- or double-stranded DNA by DNase I has a phosphate group at the 5' end and a hydroxyl group at the 3' end. Its activity is dependent on calcium ions and can be activated by magnesium ions or divalent manganese ions. In the presence of magnesium ion, DNase I can randomly shear any site of double-stranded DNA. In the presence of divalent manganese ions, DNase I shears DNA double-stranded at the same site, forming flat ends, or sticky ends with 1-2 nucleotides protruding.
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Removal of possible DNA contamination such as genomic DNA from RNA samples before RT-PCR reactions;
Removal of DNA templates after RNA transcription catalyzed by RNA Polymerases such as T7, T3, SP6, etc. in vitro;
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* This product is for research only.
