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Dispase II

RRPFR0203

Dispase II is a non-specific metalloproteinase that is often used in cell biology to isolate single cells from different tissues or organs and for subsequent cell cultures, such as primary cell isolation and cell passaging. In addition, Dispase II is used to eliminate cell aggregation that occurs during suspension cell culture. Compared with other proteases commonly used in cell biology, this enzyme has the following advantages: 1) a fast, effective and gentle cell digesting enzyme that causes little damage to the cells and maintains the integrity of the cell membranes; 2) of bacterial origin without contamination by mycoplasma or other animal viruses; 3) stable and unaffected by temperature, pH, and serum fractions; and 4) can be used for the Separation of many types of tissues and cells, etc.

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Applications
Cell culture.
Storage
Lyophilized powder can be stored at 4°C with a shelf life of 2 years. The storage solution can be stored stably at 4°C for 2 weeks. For long term storage, please dispense and freeze at -20°C, effective for 2 months. Avoid repeated freezing and thawing.
Shipping
Transportation in ice packs.
PH
6.0-8.5
Inhibitors
EDTA, EGTA, Hg2+, other metal ions. Serum does not inhibit dispase activity.
Activator
Ca2+, Mg2+, Mn2+, Fe2+, Fe3+, Al3+. The optimal Ca2+ concentration is 2 mM. The optimum Ca2+ concentration is 2 mM, and the enzyme product contains sufficient Ca2+ for optimum activity.
Sources
Bacillus polymyxa
Others
It needs to be sterilized when used, and the common working concentration for cell culture is 0.6-2.4 U/mL.

* This product is for research only.

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